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DON/trichothecene hypersensitive yeast strains for phenotypic testing of heterologous RPL3 cDNAs (conditiional RPL3 strain with pdr5 ayt1)

We have developed yeast strains that are sensitive to trichothecenes and have a conditional RPL3 gene (expressed on galactose medium and therfore viable, not transcribed on glucose medium). Such strains can be transformed with plant cDNAs encoding wildtype or mutant alleles of RPL3, and the ability of the transgene to replace the yeast gene functionally and its toxin resistance properties can be phenotypically detected.

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Institute of Applied Genetics and Cell Biology,
Muthgasse 18
1190 WIEN
Austria
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