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A DON induced wheat cDNA library

We isolated RNA from deoxynivalenol-treated roots of wheat cultivar Frontana harvested 24 h post-treatment. This RNA was used to generate a cDNA library using the Clontech Smart cDNA cloning kit. This oduced from DON-treated roots (wheat cv. Frontana) harvested 24h post-incubation. This phage library was converted to a plasmid library (pTriplEx2). The library was then normalised in order to reduce the number of redundant cDNAs. The resulting plasmids were transformed into E. coli. Upon plating of the transformed bacteria, this resulted in excess of 3,000 clones, which were amplified and stored at 70oC.


Fiona DOOHAN, (Lecturer)
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