Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

FP5

13C-STARCH Résumé de rapport

Project ID: QLK1-CT-2001-00431
Financé au titre de: FP5-LIFE QUALITY
Pays: United Kingdom

Availability of technique to measure 13C-short chain fatty acids in urine

The main focus of this work concerns a procedure to measure volatile fatty acid production in the body. We are now in a much better postion in being able to measure and relate this to potential health benefit of different diets. We use stable isotope tracers to investigate VFA production by isotope dilution. Key steps in validating this protocol have been undertaken. Firstly, we have developed and proven analytical methods. We have developed a robust GCMS method to measure the concentration and 2H-enrichment of VFA in urine and plasma.

This new method has been proven during our collaborative studies. We have ensured that the sample preparation procedure yields sufficient sample, in the correct form, for both GCMS and GC-combustion-Isotope Ratio MS (GC-C-IRMS) analysis. GC-C-IRMS is a new method for compound-specific analysis at low 13C-enrichment. This is a novel development made entirely during the Eurostarch project. Most recently we have demonstrated that our procedure can be extended to propionate analysis. We have placed this work in the public domain. A paper has been published in Rapid Communications in Mass Spectrometry, where EU funding was fully acknowledged:

Morrison et al (2004) Rapid Communications in Mass Spectrometry 18: 2593-2600.

Contact

Tom PRESTON, (Reader in Biochemistry)
Tél.: +44-135-5270108
Fax: +44-1355229898
E-mail