Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

FP5

BIOBRUSH Informe resumido

Project ID: EVK4-CT-2001-00055
Financiado con arreglo a: FP5-EESD
País: United Kingdom

Culture collection for bioremediation

THE BIOBRUSH CULTURE COLLECTION was assembled from different sources supported by information about efficacy and safe use in bioremediation. Screening covered biocalcification, hydrocarbon breakdown, denitrification and sulphate reduction. Cultures were also isolated from new sources. The information has been condensed into a database resource as Microorganism Data Sheets. Studies were done on sulphate-reducing bacteria (SRB), nitrate-reducing bacteria (NRB) and biocalcifying bacteria and Microorganism Data Sheets recorded cultural characteristics, activities, identification by molecular sequencing and level of health risk. Screening for calcite production yielded F2 for field trials, identified as Pseudomonas putida, showing low risk to humans, sensitivity to most antibiotics and precipitating calcite at almost all temperatures tested. Brevundimonas diminuta was identified as a potential organic degrader but the organic compounds in stone was mostly too low to require biological treatment so studies were discontinued. For sulphate removal, Desulfovibrio vulgaris subsp. vulgaris was chosen for field trials because of activity under non-strictly anaerobic conditions and ease of detection. For nitrate removal, two NRB environmental isolates, identified as Pseudomonas pseudoalcaligenes and Pseudomonas pertucinogena were chosen, active up to pH 10, at temperatures 10 - 15°C and having no pathogenic properties.

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UNIVERSITY OF PORTSMOUTH
School of Biological Sciences, King Henry I Street
PO1 2DY Portsmouth
United Kingdom
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