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Project ID: ICA4-CT-2001-10014
Financiado con arreglo a: FP5-INCO 2
País: Italy

Evaluation and standardisation of a rapid screening direct-plating method for detection of antimicrobial resistance in commensal microbiota of the gut

Rapid screening methods, based on the detection of faecal resistant Escherichia coli strains directly on the McConkey plates, suffered from a lack of standardisation. With the aim to fill this gap, a study was carried out to evaluate the performance of a direct-plating method (DPM) for detection of antimicrobial resistance of faecal E. coli from healthy subjects, and to compare the results with those obtained with a conventional method.

Faecal samples were obtained by rectal swabs or swabs from fresh stool from 71 healthy children aged 6-72 months from urban and rural areas of Peru and Bolivia. Microbiological analysis was performed contemporaneously by DPM and by conventional method. In the rapid screening method a faecal swab was directly plated onto McConkey agar plate and antimicrobial disks applied onto the seeded plate. The same faecal swab was also processed by standard agar disk diffusion method based on testing antimicrobial susceptibility in three randomly selected colonies from primary cultures of faecal samples on selective media. Raw data from rapid screening method were obtained by the direct reading of the plate and subjected to confirmatory analysis.

A good concordance between the DPM and conventional method was observed in detecting carriage of resistant E. coli with a higher sensitivity of the DPM. The analysis of results allowed to define interpretive criteria for DPM raw data. Applying these criteria, DPM showed good sensitivity and specificity at very low cost (ten times cheaper than the conventional method). The DPM proved to be a very sensitive, specific, fast and cheap procedure to investigate the faecal carriage of drug-resistant E. coli and may represent an useful tool to conduct large-scale resistance surveillance studies and to monitor resistance-control programs cost-effectively, particularly in low resource countries.

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Contacto

Alessandro BARTOLONI, (Associate professor)
Tel.: +39-055-7949482
Fax: +39-055-7949480
Correo electrónico
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