Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

Method for preparation of cell free Human Herpes Virus-8 (HHV-8) inoculum

A novel system for producing HHV-8 cell free virus inoculum was established. This was obtained from activated BC3 cells and subsequent purification and concentration by high-speed centrifugation.

The second step was in identifying a continuous cell line supporting viral production. The experiments showed that Vero cells are permissive to HHV-8 replication, and efficiency of virus production is similar (even higher) than that of primary endothelial cells, a well-recognized cell target of HHV-8 infection.

We are trying to enhance the system, by production of Vero cell clones stably transformed with ORF50, a key promoter of HHV-8 replication.

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Section of Microbiology
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