Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

FP5

ANTICANCER RETINOIDS Berichtzusammenfassung

Project ID: QLK3-CT-2002-02029
Gefördert unter: FP5-LIFE QUALITY
Land: Italy

Set up and characterization of cell systems supporting Human Herpes Virus-8 (HHV-8) infection

It was determined that several cell lines are susceptible and permissive to exogenous HHV-8 infection.

The cell types suitable to conduct HHV-8 in vitro infection include: human endothelial cells from umbilical cord (HUVEC, a primary cell culture representing one of the natural targets of in vivo HHV-8 infection), Vero (a continuous cell line derived from monkey kidney and permissive for several human viruses), 293 (a human kidney epithelial derived continuous cell line), ECV-304 (a human cell line presenting markers specific of endothalial cells), U937 (a monocytic cell line) and primary human macrophages from healthy blood donors.

HHV-8 did not induce any lytic cytopathic effect, but in HUVEC cells induced a typical spindle shape phenotype. All cell lines supported lytic replication, as shown by analysis of viral transcription, and by release on infectious virus in culture supernatant.

Reported by

Section of Microbiology
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