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Assay to detect Ovalbumin-specific IgE in the serum

A new ELISA protocol for detection of Ovalbumin-specific IgE antibody in serum has been developed.

The wells of a polistyrene plate were coated overnight with anti-IgE Ab. Following blocking, various dilutions of the serum were added to the plate.

After 1h incubation with biotinylated Ovalbumin followed by 1h incubation with streptavidin bound to alkaline posphatase, plates were developed using specific substrate.

The absorbance at 405 nm was determined by an ELISA reader.

Reported by

Consiglio Nazionale Ricerche
Istituto di Genetica A. Buzzati Traverso, Via P. Castellino, 111
75010 Napoli
Italy