Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

FP5

ALLDNAVAC Résumé de rapport

Project ID: QLK3-CT-2002-02026
Financé au titre de: FP5-LIFE QUALITY
Pays: Italy

Assay to detect Ovalbumin-specific IgE in the serum

A new ELISA protocol for detection of Ovalbumin-specific IgE antibody in serum has been developed.

The wells of a polistyrene plate were coated overnight with anti-IgE Ab. Following blocking, various dilutions of the serum were added to the plate.

After 1h incubation with biotinylated Ovalbumin followed by 1h incubation with streptavidin bound to alkaline posphatase, plates were developed using specific substrate.

The absorbance at 405 nm was determined by an ELISA reader.

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Anna RUFFILLI, (Head of Unit)
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