Service Communautaire d'Information sur la Recherche et le Développement - CORDIS

FP5

ALLDNAVAC Résumé de rapport

Project ID: QLK3-CT-2002-02026
Financé au titre de: FP5-LIFE QUALITY
Pays: Italy

pCMVOva DNA immunization plasmid containing the ova gene, encoding for the ovalbumin allergen

Plasmid pCMVOva is an in-house developed construct containing the ovalbumin gene, to be used for cell transfection and in vivo immunization experiments.

In this plasmid, the ovalbumin gene is cloned as fusion to the tPA leader sequence, in order to allow Derp1 secretion by transfected cells. The plasmid is able to express the encoded antigen in vitro-transfected cells.

Furthermore, the plasmid is capable of inducing allergen-specific cellular and humoral responses in non-allergic mice. The construct is particularly designed for DNA immunization experiments aimed at evaluating the immunogenicity of DNA-based allergy vaccine in murine models of naïve and allergic mice.

The plasmid is suitable for cell transfection assays aimed at studying allergen expression and presentation by antigen presenting cells and cell activation pathways upon DNA uptake. The application can be disseminated as further R&D.

Reported by

Novartis Vaccines and Diagnostics Srl (ex Chiron)
Via Fiorentina, 1
75010 Siena
Italy
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