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FP5

ALLDNAVAC Sintesi della relazione

Project ID: QLK3-CT-2002-02026
Finanziato nell'ambito di: FP5-LIFE QUALITY
Paese: Italy

pCMVDerP1 cyt DNA immunization plasmid containing the gene encoding for the dust mite allergen of Dermatophagoides pteridissinus (DerP1)

Plasmid pCMVDerp1 cyt is an in-house developed construct containing the Der P1 gene, to be used for cell transfection and DNA immunization purposes.

In this plasmid, DerP1 is cloned directly downstream from the CMV promoter, in order to avoid Derp1 secretion and retain Derp1 expression in the cytoplasmic compartment of transfected cells.

The plasmid is able to express the encoded antigen in vitro transiently transfected cells. This construct is particularly suitable for in vitro transfection studies aimed at evaluating Derp1 processing and presentation and to investigate cell activation pathways upon DNA uptake.

The plasmid is also suitable for DNA immunization purposes, to study plasmid immunogenicity in the murine models of naïve and allergic mice. The application can be disseminated as further R&D.

Informazioni correlate

Contatto

Guido GRANDI, (Head Biochemistry and Molecular Biology Unit)
Tel.: +39-0577-243506
Fax: +39-0577-243564
E-mail