Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

Hydrophilic biochip surfaces

Hydrogel surfaces based on polyurethane (PU) and poly(vinyl alcohol) (PVA) modified with anionic, cationic and neutral additives (e.g. RL100 (Meth1), RS100 (Meth2), E100 (Meth3), Praestol 187, Praestol 185, Praestol 190, Polystabil KWS, chitosan) and crosslinkers (e.g. polyethylenimine (PEI), polyallylamine (PAH), glutaraldehyde, phenylendiamine (HAD), trianzinyl-ß-cyclodextrin (MCT), cyanurchloride (TsT) have been developed and tested for protein immobilization.

Best-of surfaces have been selected with respect to signal-to-noise ratio and data reproducibility (CV <40%). The surfaces have been characterized with regard to biologically relevant parameters (signal-to-noise ratio S/N, % background, immobilization capacity) and by physical methods (AFM done at BGU and ellipsometry at IREE) and compared with ARChip Epoxy and the poly(acrylamide) slide from Perkin Elmer.

Data from contact angle measurements and swellability studies, furthermore, topographic and roughness data from AFM were correlated with S/N values to design a high quality protein surface. PU1 was used as platform in an on-chip immunoassay for 17ß-estradiol. The dynamic range was from 0.001 to 0.014 µg/ml 17ß-estradiol.

Reported by

ARC Seibersdorf research GmbH
Seibersdorf
2444 Seibersdorf
Austria
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