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Highly multiplexed SPR sensor

A new concept of a high-throughput surface plasmon resonance (SPR) sensor based on the angular spectroscopy of surface plasmons on a two-dimensional array of diffraction gratings was developed and a laboratory prototype (breadboard level) of the sensor was realized.

In this sensor, SPR angular spectra from a single row of diffraction gratings are acquired using a two-dimensional detector and the reading of SPR spectra from rows of diffraction gratings is performed sequentially by chip scanning. The special chip with an array of diffraction gratings is integrated with a sensor chip cartridge interfaced with a multi-channel flow-cell.

In model refractometric experiments, the sensor sensitivity to refractive index changes was found to be about 160 deg/RIU (refractive index unit). The minimum detectable change in the refractive index was determined to be about 7x10E-7 RIU. This sensor presents a generic platform offering a very high number of independent sensing channels (up to several hundreds).

In conjunction with appropriate surface chemistries and biorecognition elements it could be applied to parallelized observation of molecular interactions or detection of large number of analytes or screening of a large number of samples for a single analyte.

Informations connexes

Reported by

ARC Seibersdorf research GmbH
Forschungszentrum Seibersdorf
2444 Seibersdorf
Austria