Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

Development of functional viral expression vectors encoding core and envelope genes of MVV for use as immunogens and making recombinant proteins

The genes encoding MVV EV1 p55gag precursor and gp160 env precursor were cloned and ligated into a commercially available live attenuated adenovirus vector that express the recombinant proteins in mammalian cells in vitro and in vivo. In addition, the gene encoding MVV EV1 p55gag precursor was cloned and ligated into a commercially available modified vaccinia Ankara (MVA) live attenuated virus vector. An MVA vector encoding the MVV gp150 env precursor was already available before the project started and represents prior knowledge. The adenovirus vectors encoding p55gag or gp150env were unstable and require further manipulation to render them functional. The MVA vectors are functional. These recombinant vectors can used as immunogens for priming or boosting in vaccine studies (as in this project), or for studying the function of the recombinant proteins in cells, or for producing recombinant p55gag or gp150env precursor recombinant proteins in mammalian cells.

The end users of the information are other scientists working in the field of SRLV control, or working with other lentiviruses, or working with other plasmid DNA vaccines, or in the general area of vaccines and immune responses, or in the general area of lentivirology.

Commercial companies may be interested in the MVA vectors, but until an effective vaccine strategy is produced it is unlikely that there would be a market for the vectors other than for the SRLV research community, which is very small. Similarly, using these vectors to produce recombinant proteins for diagnostic purposes is unlikely since the amounts produced would be too small to be commercially viable. Also, the gag components p25, p17, and p14 can all be produced in large quantities using bacterial expression systems. The gp150 protein would be of potential interest for diagnostic purposes, but the amounts that could be produced are likely to be too small.

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