Servizio Comunitario di Informazione in materia di Ricerca e Sviluppo - CORDIS

Salmonella aborts ovis Rv6 and S. typhimurium do not support functional expression of eukaryotic plasmids encoding MVV genes in vitro or in vivo

Salmonella abortus ovis Rv6 was found not to support stable transformation of eukaryotic expression plasmids encoding MVV gag and env genes. When the plasmids were conditioned by prior transformation into a low endonuclease strain of S. typhimurium, stable transformation of the Rv6 strain was achieved. However, no expression was observed from these plasmids in vitro or in vivo. An Aro- strain of S. typhimurium also failed to give expression of the transgenes. It was concluded that the plasmids used were not functional inside the salmonella strains chosen for the work, and that further work would have to be performed to identify suitable plasmid/salmonella strain combinations that would give expression.

The end users of the information are other scientists working in the field of SRLV control, or working with other lentiviruses, or working with other plasmid DNA vaccines, or in the general area of vaccines and immune responses, or in the general area of lentivirology, or in the general area of bacterial vectors. Commercial companies may be interested in the information though they are unlikely to consider commercialisation.

Reported by

AGENCE FRANCAISE DE SECURITE SANITAIRE DES ALIMENTS
ROUTES DES CHAPPES, 105B.P. 19
06902 SOPHIA ANTIPOLIS
France