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REDALL Informe resumido

Project ID: QLK1-CT-2002-02687
Financiado con arreglo a: FP5-LIFE QUALITY
País: United Kingdom

Rapid methods for allergen determination

Improved methods for detection of allergens are urgently required by the food industry in order to provide enhanced risk assessment. Highly sensitive and rapid tests have been developed for the detection of trace amounts of food allergens from animal origin in various food matrices.

Such methods include dipstick and biochip sensor (based on resonance-enhanced nanoclusters) technologies, which can be applied for the detection of allergens in the raw materials, intermediate products and final food products during the food manufacturing process. The methods developed are sensitive, cost-effective, easy-to-use, robust and reliable, and can be completed in minutes rather than hours.

The rapid DNA extraction procedure for PCR analysis takes about 30 min while the CTAB extraction procedure takes more than 3 hours. The limits of detection were suitable for the detection of meat (beef and chicken meat) and skimmed milk powder in various samples. The limits of detection were one order of magnitude higher than for the CTAB extraction method.

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Mike MORGAN, (Director)
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