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Identified and characterised CSFV specific T-cell epitopes

We have demonstrated that CSFV genes Erns, E2 and NS3-4B are targets for the cellular immune system and further analysis is needed with cytotoxic T-cell (CTL) assays. In order to obtain stable clonally cell lines expressing the genes of interest, E2, Erns, NS3-4B, which are based in the vector pEVHis, are transfected into MAX cells. Since E2 appeared to be an important target for CTLs we are interested which domain will be recognised within this E2 domain. In order to determine which domain in the E2 domain is recognised by the CTL, also a clonally cell line expressing E2 with a deleted A domain was obtained. The obtained results indicated that the BC domain of E2 most likely contains important CTL epitopes. Unfortunately, a clonally cell line expressing E2 with a deleted BC domain could not be made. Further experiments will be needed to elucidate this.

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