Servicio de Información Comunitario sobre Investigación y Desarrollo - CORDIS

Methods for identifying peptide bound to porcine MHC class 1 proteins

Generation of porcine MHC class 1 tetramers specific for CSFV. MHC class 1 tetramers are used to identify peptide specific CTLs. If the peptides are derived from a virus the tetramers can be used in fluorescence activated cell sorting to analyse populations of virus specific T cells isolated following infection and/or vaccination.

The CTLs that bind fluorescent tetramers can be conuter stained with antibodies against lymphocyte surface proteins and the phenotype of the cells recognising infected cells can be determined with precision. This project has produced reagents that will allow this powerful tetramer technology to be used to study pig CTLs produced during infection and or vaccination. The production of tetramesr requires the correct combination of MHC class 1 protien and peptide.

Work in VI (a) above identified a series of 9mer peptides derived from CSFV proteins that are recognised by CTLs of dd pigs infected with CSFV. These petides can now be mixed with recombinant MHC class 1 proteins and a-2 microgobulin (described in V11I-IX) below to make tetramers with the potential to identify CTLs specific for CSFV. This will allow the surface phenotype of CSFV-specific CTLs and the peptides they recognise to be determined with precision.

Contacto

Tiny DE BRUIN, (Project Coordinator)
Tel.: +31-32-0238238
Correo electrónico
Síganos en: RSS Facebook Twitter YouTube Gestionado por la Oficina de Publicaciones de la UE Arriba