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Characterization of oocyte cathepsins and their enzymatic activities during oocyte development

1. Cloning and expression studies of ovarian cathepsin mRNA by Real Time PCR.
2. Optimisation of ovarian cathepsin enzymatic assays.
3. Determination of cathepsin mRNA expression during ovarian maturation.
4. Determination of cathepsin enzymatic activity during oocyte maturation.
5. Studies on the relationship between in vitro oocyte maturation, cathepsin activity and hydration.

Reported by

UNIVERSITA POLITECNICA DELLE MARCHE
DIPATIMENTO DI SCIENZE DEL MARE, UNIVERSITA POLITECNICA DELLE MARCHE, VIA BREECE BIANCHE
60131 ANCONA
Italy
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