Community Research and Development Information Service - CORDIS

Real time PCR methods for 14 key genes in salmon, sea bass and rainbow trout

Real-time quantitative PCR assays have been developed, validated and applied for gonadotropin subunits (GTHa, FSHb and LHb) both in salmon and sea bass, for GnRH-R's in sea bass and trout (type I and type II), different ER's (ERa, ERb1 and ERb2) in trout, and FSHR, LHR and AR's (a and b) in salmon. The mRNA expression profiles of these genes have been put into the context of pubertal and/or seasonal development, as well as photoperiod treatments, in both male and female sea bass, rainbow trout and Atlantic salmon, by correlating the expression profiles with the stage of gonadal development (stage of spermatogenesis or oogenesis) and plasma sex steroid profiles.

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INSTITUTE OF MARINE RESEARCH
1870 NORDNES
5817 BERGEN
Norway
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