Forschungs- & Entwicklungsinformationsdienst der Gemeinschaft - CORDIS

FP6

BIRA FOR CHIP-CHIP Berichtzusammenfassung

Project ID: 40337
Gefördert unter: FP6-MOBILITY
Land: United Kingdom

Final Activity and Management Report Summary - BIRA FOR CHIP-CHIP (Optimisation of a non-antibody based technology to improve the efficiency of genome-wide analysis of DNA binding sites)

Now that the human genome is fully sequenced, a current goal is to study proteins important in controlling gene expression. These proteins ensure that genes are expressed in the right cell type, at the right level, and at the right time. Many of the proteins which control gene expression bind to DNA sequences in the genome - thus providing a framework for cellular responses which regulate the activity of genes nearby. Since current DNA-binding protein based strategies are dependent on specialised affinity reagents - antibodies - the present proposal aimed to overcome the requirement for specific antibodies for each and every different protein which are to be studied.

This is accomplished by attaching a biotin "tag" onto the proteins which control gene expression. This is accomplished by altering the genes for these proteins so that they contain a biotin "tag". When these proteins are expressed, this "tag" became part of the proteins themselves. One can then isolate the "tagged" protein using existing protein purification techniques. This method allows the genome locations of these proteins and the genes that they regulate to be determined. During the Marie Curie post-doctoral training period, the applicant Serdar Kasakyan learned a state-of-the-art technique (Bacterial Artificial Chromosome recombineering) at the Technical University of Dresden and set up this approach in the host laboratory (Dr David Vetrie) at the University of Glasgow.

By using this technology the applicant constructed biotin tagging vectors for four proteins which bind DNA and regulate gene expression (TAL1, LDB1, JHD1A, NSD1). These vectors will be placed into cells and will convert normal copies of the genes for these proteins into "tagged" versions. Cell lines containing these "tagged" proteins are currently being generated and will be a powerful set of tools for further studies in the host laboratory aimed at investigating gene regulation. Thus, the applicant has facilitated both his own training in this state-of-the-art field, as well as facilitating the research in the host laboratory.

Kontakt

David VETRIE, (Principal Investigator)
Tel.: +44-141-211-2233
Fax: +44-141-330-5611
E-Mail-Adresse
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