Community Research and Development Information Service - CORDIS

Advanced compounds for effective cell transfection

Oligomer compounds display increased potential to destabilise cell membrane and thus allow cell transfection by highly specialised substances for experimental or clinical purposes. Urged by this, a French company recently developed a particular structure of oligomer that are capable of achieving reliable and high specific cell transfections.
Advanced compounds for effective cell transfection
The synthesis of oligomers involves joining of simple compounds to form polymers, called monomers and this polymer normally comprises more than two monomers. These valuable compounds have increased capabilities under specialised conditions to permeate through the cell membrane and transfect cells with various substances. Such substances include oligonucleotides, peptides or oligosides, which are widely used in many experimental and clinical applications.

This newly developed method allows the preparation of a particular structure of oligomer that displays a low degree of polymerisation. More than half of this structure is substituted with molecules inducing a membrane destabilisation in acidic medium and thus increasing cell permeability. This novelty specifically addresses the complex oligomer-molecules to be transfected towards specific cells for the transfer of the biomolecules in cytosol and/or nucleus of the cells.

When targeting specific tissues or cells, the produced oligomer conjugates display increased specificity since they include molecules acting as recognition signals to be recognised by membrane cellular receptor. Transfection experiments carried out on a type of human cell lung carcinoma proved that these compounds may result in a high reliable and efficient transfer of oligonucleotides into cells. Exhibiting low toxicity, these compounds may be used for cell transfection for in vitro, ex vivo, in vivo, and medical purpose.
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