Community Research and Development Information Service - CORDIS

Regenerated sensors

The attachment of molecules on solid substrates has many biological applications including biosensing, medical implants, and control of cell adhesion and growth. A French team has invented a process of non-covalent attachment of various kinds of ligands on solid substrates. The main element of this process is the creation of sensors that can be regenerated.
Regenerated sensors
Sensors are an important tool in biotechnology that is used in conjunction with analysis techniques such as spectroscopy for the detection of specific elements. Sensors are used in many applications for the detection or determination of various types of elements such as antigens or antibodies. Biosensing technology has extended its range of applications and has improved its capabilities due to innovations in biotechnology. The attachment of molecules on solid substrates is one of these applications.

A French research team has introduced an innovative process of non-covalent attachment of a protein, DNA or specific fragment on solid substrates such as slides or beads. The reversible character of the non-covalent binding allows the release of immobilised molecules under soft conditions. Due to this property the initial surface could be completely regenerated and can be used for new experiments having exactly the initial capabilities and characteristics required.

This process could find excellent application in the development of sensors that are using immobilised molecules. These sensors in cooperation with optical or spectroscopic detection techniques are used for the detection of specific antigens or antibodies, sDNA fragments and carbohydrates. After their use these sensors could be regenerated.

It is important that the process allows an easy way for the control of ligand density and reproducibility. In the future the process could be further extended to many kinds of ligand such as antigens, polysaccharide, drug and hormone.

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Record Number: 81036 / Last updated on: 2005-09-18
Domain: Biology, Medicine