Community Research and Development Information Service - CORDIS

Multiplex sequencing method in one single reaction

MULTIseq is a novel sequencing method of highly complex mixtures of nucleotide acids in one single reaction. This simple and cost-efficient method may find useful applications in clinical diagnostics as well as in biotechnology and environmental analyses.
Multiplex sequencing method in one single reaction
Unlike most common multiplex sequencing approaches MULTIseq does not require any additional restrictions in primer design or reaction conditions. This real multiplex sequencing technology comprises testing of any sample including nucleotide acid material coming from various sources. Two examples are a human clinical sample for screening for high risk subtypes of a human virus or disease and an environmental sample for analysis of microorganisms.

With the aid of the MULTIseq technique a complex mixture of known nucleic acids is sequenced in one tube following a single sequencing reaction step performed in state-of-the-art thermocyclers. The newly developed method leads to both qualitative and quantitative specification of every single nucleic acid sequence. MULTIseq also displays compatibility with other sequencing methods such as pyrosequencing approach that is mostly used by clinical researchers for analysis of genetic mutations.

Furthermore, it may also work with classic sequencing reactions if commercial capillary sequencers are specifically adapted. The method has already been used for the analysis of microorganisms in environmental biotechnology studies, but it shows increased potential for clinical applications. Thereby, the process is suited for detecting and quantifying differential gene expression patterns in tissue samples as a genetic “fingerprint” of types of a disease such as a tumour.

Apart from simplicity the innovative technique features robustness, cost-efficiency, reliability and speed. Biotech companies and/or manufacturers of analysing instrumentation are sought for technical co-development and implementation of the new technology within company's own instruments (thermocyclers) or procedures. Once the technology is implemented a licensing agreement may be reached if necessary.
Record Number: 81544 / Last updated on: 2005-09-18
Domain: Biology, Medicine