Community Research and Development Information Service - CORDIS

Horizon 2020 project information and now also report summaries are available on CORDIS. All H2020 projects can be downloaded from the EU Open Data Portal .

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Identification of BAC clones encoding enzymes involved in polysaccharide breakdown from a metagenomic library of DNA from the rumen of cattle
The ability to screen metagenomic libraries containing large inserts for potentially valuable enzymes and other microbial gene products has been examined using an available BAC (bacterial artificial chromosome) library of DNA inserts from the rumen contents of cattle. Average ...
Programme: FP5-LIFE QUALITY
Record Number: 44423
Last updated on: 2007-08-22
Novel biocatalyst genes from metagenomic DNA libraries
Method for the direct isolation of environmental DNA (metagenome DNA) from various habitats has been developed. Subsequently, metagenomic DNA libraries have been constructed and several screening procedures have been developed. The libraries are rich sources for the isolation ...
Programme: FP5-LIFE QUALITY
Record Number: 44422
Last updated on: 2007-08-22
Mining different classes of P450s
Primers and protocols for the amplification of different classes of P450s from actinomycetes have been developed. PCR products have been sequenced and analysed. Genome cosmid libraries have been constructed for selected actinomycetes and the full-length genes have been sequenc...
Programme: FP5-LIFE QUALITY
Record Number: 44421
Last updated on: 2007-08-22
Recombinant S. lividans strains that efficiently secrete protein of interest via the Sec pathway
In first instance, the use of the newly developed pSSV05 in order to obtain efficient heterologous protein production by the streptomycete host, was evaluated by inserting existing expression/secretion cassettes. These cassettes consist of the promoter and signal sequence of t...
Programme: FP5-LIFE QUALITY
Record Number: 44420
Last updated on: 2009-03-13
Suitable cloning vector for DNA transfer to Streptomyces
A conjugative E. coli/Streptomyces vector was developed using the ori/rep functions of E. coli plasmid pUC18 and Streptomyces plasmid pSVH1, and the oriT locus of the broad host range conjugative vector RK2. Additionally, for selection reasons the apramycin resistance gene fun...
Programme: FP5-LIFE QUALITY
Record Number: 44419
Last updated on: 2007-08-22
Expression of Streptomyces lividans isoamylase in Bacillus
Two plasmid encoding putative isoamylase genes were obtained from partner Combinature. The respective genes were cloned in B. subtilis and over-expressed at low levels. Characterization showed, that the proteins showed no isoamylase activity and hence the work on these enzymes...
Programme: FP5-LIFE QUALITY
Record Number: 44418
Last updated on: 2007-08-22
Functional screening of metagenomic libraries for industrial relevant enzymes
One of the major tasks of Novozymes in the GEMINI project was to identify novel cellolytic enzymes from metagenomic source. One approach to get hold of the immense diversity is by functional screening genomic libraries on indicator substrates. The initial screening of the meta...
Programme: FP5-LIFE QUALITY
Record Number: 44416
Last updated on: 2007-08-22
Quality control of metaGenomic libraries by E.coli complementation
The aim of this work is to analyse the biodiversity and thereby quality of metagenomic libraries before the set-up of functional- and PCR-based screening assays. For this purpose, functional complementation of an E. coli purine synthesis mutant (delta purD) was set up. Genes e...
Programme: FP5-LIFE QUALITY
Record Number: 44415
Last updated on: 2007-08-22
Cloning of M4 protease in Bacillus
Novozymes has obtained a plasmid containing a family M4 protease (thermolysin like) from GEMINI partner 7 (R. Daniel, GEORG-AUGUST-UNIVERSITY GOETTINGEN). The gene was identified by them via screening of a activated sludge metagenomic library on casein plates. The enzyme was d...
Programme: FP5-LIFE QUALITY
Record Number: 44417
Last updated on: 2007-08-22
Isolation of the S. lividans phage shock protein (psp) A gene and the effect of its over-expression on protein secretion
DeLisa et al. (2004) described that pspA overexpression resulted in a 3-fold increase of Tat-dependent secretion in E. coli. In S. lividans, a pspA homologue was found, but no homologues of pspB and pspC genes, thought to be needed for a functional phage shock-protein (psp) re...
Programme: FP5-LIFE QUALITY
Record Number: 44414
Last updated on: 2007-08-22
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List retrieved on: 2017-02-23