DISSECTION OF AN ARCHAEAL RNA POLYMERASE
|Host Laboratory||Scientific Supervisor|
| Max-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
Institut fur Biochemie
AM KLOPFERSPITZ 18A
82152 Martinsried bei Munchen
| Prof. Wolfram Zillig |
Tel : 49 089 85782229 / Fax : 49 089 8578 27 28
Email : firstname.lastname@example.org
| Mr. Kenneth Mark Stedman (British) |
Tel : / Fax :
In order to better understand the basic and fundamental aspects of eukaryal (also known as eukaryotic) and archaeal (also known as archaebacterial) transcription, an archaeal RNA polymerase will be reconstituted using recombinant subunits. The archaeal RNA polymerase is a paradigm for all multi-subunit RNA polymerases, and probably closely resembles the first RNA polymerases. It is also simple in that it requires only two general transcription factors for accurate transcription and binds DNA specifically in the absence of other factors. Reconstitution with recombinant subunits will be the first for multi-subunit RNA polymerases and should allow fundamental insight into the structure and function of both archaeal and eukaryal RNA polymerases and their subunits. The interactions of the individual subunits with each other, with the basal transcription factors and with DNA will be investigated by sequential reconstitution, protein-protein and DNA-protein cross linking experiments.
Contract number : BIO4CT965111
Last updated :20-04-1999
Do you have questions or comments about this site : Lifeemail@example.com