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EXPRESSION OF VOLTAGE-SENSITIVE K+ CHANNELS FOR STRUCTURAL STUDIES |
| Host Laboratory | Scientific Supervisor | |
| European Molecular Biology Laboratory MEYERHOFSTRABE 1 102 209 69012 Heidelberg Germany |
Dr. Werner Kuhlbrandt Tel : 49/6221 387245 / Fax : 49/6221 387306 Email : KUEHLBRANDT@EMBL-HEIDELBERG.DE | |
| Grant Holder | ||
| Mr. David Nicholas Parcej (British) Tel : 44/171 594 5241 / Fax : 44/171 225 09 60 Email : D.PARCEJ@IC.AC.OK | ||
| Abstract Mamba venoms have allowed the characterisation and purification of certain voltageactivated K+ channels from brain tissues and allowed their subunit and oligomeric structures to be described (see Proposal) A more detailed elucidation of the K+ charmed structure, including definition of secondary structure elements and components involved in K+ channel function (e.g. voltage sensing, inactivation and activation) will require the production of 2- and, in the long-term, 3-dimensional crystals. While using the purified, natural K+ channels may be desirable, producing enough material for such studies is a considerable undertaking. Thus, the aim of this proposed study is to systematically evaluate a number of systems for the overexpression of both alpha and beta subunits- singly and together- in an active, native form. When a suitable system has been found, 2-dimensional crystallisation trials will be performed, followed by electron microscopic analysis. Contract number : BIO4CT965117 | ||
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