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STUDIES ON RIPENING_SPECIFIC GENE EXPRESSION IN APPLE USING THE BIOLISTIC PDS-1000/HE DEVICE |
| Host Laboratory | Scientific Supervisor | |
| Horticulture Research International East Malling ME19 6BJ West Malling - Kent United Kingdom |
Pr. James David Tel : +44/1 732 843833 / Fax : +44/1 732 849067 Email : DAVID.JAMES@HRI.AC.UK | |
| Grant Holder | ||
| Mr. Stefano Biricolti (Italian) Tel : 39/55 36 16 88 / Fax : +39 55 33 14 97 Email : dobiri@cesit1.unifi.it | ||
| Abstract Research objectives and content Transgene expression in apple plants is currently driven by constitutive promoters. Therefore, expression occurs m all tissues in an untargeted manner. For the production of genetically improved apples, it will be vital to target the effects of transgene activity by the use of tissue-specific promoters. Two ripening-specific promoters have recently been isolated at HRI-East Malling. Transgenic apple plants are being produced in which these promoters drive a marker gene (gas) to permit analysis of gene expression in the fruit. Because of the time it will take before the plants bear fruit, it is planned to use particle bombardment to directly introduce promoter construct DNA mto apple fruit tissues to produce transient gas expression. A Biolistic device has recently been obtained at HRI-East Malling for this purpose. It is intended that this study will be used to demonstrate that particle bombardment is a suitable method for examining promoter activity in apple fruit tissues, and to optimise parameters to achieve the highest levels of transient gene expression. Once the methodology has been established, this will permit later dissection of fruit-specific promoters to identify minimal promoter cassettes for use in genetically improved apple plants of the future Training content (objective, benefit and expected impact) Biolistics PDS-1000/He is a very expensive biolistic device. At HRI laboratory such device is available along with an experienced staffworking on fruit trees. Expertise is therefore available to provide theoretical and practical guidance on all the procedure described in the proposal. The 3 month period of study for which this application is made would be very important for a University teacher and researcher. Training will be given in the use of the Biolistic PDS-1000/He device and in biochemical and molecular analysis techniques used to examine transient gene expression produced by particle bombardment. Links with industry / industrial relevance (22) This study will be pure research but the findings may have relevance to the apple industry in the future. The Apple and Pear Research Council raise a levy from growers to aid research. The host institution receive funding directly from the APRC. Contract number : FAIRCT965071 | ||
Last updated :22:01:98
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