CORDIS Archive : Training and Mobility of Researchers / Contract Nr : FMBICT950465

Host Laboratory	Scientific Supervisor
Danmarks Tekniske Universitet Department of Biotechnology BLOCK 223 DTU 2800 Lyngby Denmark	PROFESSEUR Jens Nielsen Tel : 45/45252660 / Fax : 45/45884148
	Grant Holder
	MR. Christopher Klein (German) Tel : 45/35262316 / Fax :
Abstract Glucose repression in yeast represents a major problem for both the cultivation on industrial substrates and for the breadmaking (leavening) because glucose has to be depleted before the remaining sugars can be utilized. In glucose-derepressed Saccharomyces cerevisiae strains, simultanous, and concomitantly more time-efficient, utilisation of the sugars would be possible resulting in an improved process economy and a lower environmental burden beside better leavening characteristics. Several genes playing key roles in catabolite repression have been deleted in an industrial strain. The physiology of the recombinants will be compared with the wild type in well controlled high performance bioreactors in order to elucidate the respective gene functions in the regulatory cascade of catabolite repression. Measurements of extracellular sugars and metabolites, assays for sugar-cleavaging enzymes and uptake systems, Northern blotting and radioactive techniques will be the analytical tools providing the basis for a metabolic flux analysis. Contract number : FMBICT950465 Duration : 30 Months from 01-02-96 -- Category 20 (B20)

Last updated :10:07:98

Do you have questions or comments about this site : tmr-grants@dg12.cec.be