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HIERARCHICAL CONTROL OF DENITRIFICATION |
| Host Laboratory | Scientific Supervisor | |
| Vrije Universiteit Amsterdam Faculteit der Biologie Department of Microbial Physiology DE BOELELAAN 1087 1081 HV Amsterdam The Netherlands |
MR. Rob Van Spanning Tel : 31/204447179 / Fax : 31/204447229 Email : spanning@bio.vu.nl | |
| Grant Holder | ||
| MR. Neil Frank William Saunders (British) Tel : 44/1865275242 / Fax : 44/1865275259 Email : nfws@bioch.ox.ac.uk | ||
| Abstract Research objectives and content The aim of the project is to obtain a comprehensive understanding of the factors that control the expression and activities of the enzymes synthesised during denitrifying growth in the bacterium Paracoccus denitrificans. Specifically, the roles of two previously identified but poorly characterised genes, nirI and nosR are to be investigated. These genes and their products will be characterised by both genetic and biochemical methods, and their interactions with other regulatory components will be analysed using mutant strains, in combination with powerful techniques for metabolic analysis. The project will utilise a combination of molecular biology, biochemistry and biomathematics in order to address this important biological topic. Training content (objective, benefit and expected impact) The programme will provide extensive training in modern molecular biology, biochemistry and microbiology, which will both extend my current knowledge of these fields and introduce several new components, in the subjects of biomathematics and metabolic analysis for which the host institute is renowned. Links with industry / industrial relevance (22) No immediate links with industry or industrial relevance, but the work may well aid the development of microbial waste-water treatment plants which minimise the release of atmospheric pollutants (see detailed project proposal). Contract number : FMBICT972594 | ||
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