The overall objective of MASCOT is to exploit breakthroughs at the confluences of micro-, nano- and bio-technologies to create a low-cost minimally-invasive intelligent diagnosis system using a nanotechnology-based device for the isolation, enrichment and detection of rare cells from complex mixtures and using an array of nano-biosensors, integrated with micro-scale amplification, for the consecutive RNA/DNA analysis of the isolated rare cells.
Advances in molecular biology and biosensor technology and the integration of nanostructured functional components in macro- and microsystems will facilitate the isolation of rare cells on the basis of novel markers, cell size, tailored surface chemistry and labeling with magnetic particles. The advantages of the exploited biosensors for RNA/DNA analysis are their sensitivity, their inherent selectivity, their versatility and their cost effectiveness. Addressing the health care requirement of the future of an individualised theranostic approach, the specific applications that will be demonstrated in MASCOT will be the isolation and RNA/DNA characterisation of both lung and breast cancer cells (more specifically, circulating tumour cells or CTC's), from respectively sputum and peripheral blood or bone marrow.
The radical innovation proposed in MASCOT will result in a concrete prime deliverable of a technology platform of wide application and unquestionable socio-economic benefit, increasing European competitiveness whilst contributing considerably to the quality of life of the population and control of health care cost.
Funding SchemeSTREP - Specific Targeted Research Project
1081 HV Amsterdam