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PLANT RESPONSE to ‘friend or foe’: a CELL type specific nuclear proteomics approach

Descripción del proyecto

Cómo distinguen los diferentes tipos de células vegetales a un amigo de un enemigo

Las respuestas de las plantas a «un amigo o un enemigo» están reguladas en sus diferentes tipos de células. Por lo tanto, en el proyecto PlantCellResponse, financiado con fondos europeos, se empleará una novedosa técnica de marcado de proximidad de proteínas «in vivo» a fin de investigar las respuestas a bacterias beneficiosas o patógenas en la especie vegetal modelo «Arabidopsis thaliana». La persona beneficiaria de la beca de investigación determinará las respuestas de las plantas a bacterias promotoras del crecimiento vegetal (PGPB, por sus siglas en inglés), los procesos o metabolitos especializados implicados y las proteínas reguladoras que los controlan. También se investigará la regulación de la respuesta a PGPB y a un patógeno en tipos de células radiculares específicas mediante la generación de datos del proteoma nuclear específico del tipo celular. Por último, en el proyecto PlantCellResponse se caracterizará la función de candidatos a proteínas reguladoras específicas y su tipo de célula diana a través de experimentos individuales de gen por gen.

Objetivo

Plants respond to and must distinguish between beneficial and pathogenic bacteria (‘friend and foe’) surrounding them. Bacteria may manipulate the plant’s immune system to allow colonialization or can be resistant to plant defence responses such as the biosynthesis of specialized metabolites. It has been increasingly shown that plant responses occur in a cell type specific manner but single cell research has not yet been extensively explored in plant. Here, I propose to apply a state of the art in vivo protein proximity labelling technique to investigate responses to beneficial and pathogenic bacteria in the Arabidopsis thaliana root including the cell type specific level. The first objective of the proposed research is to determine if and to which extent there are differential plant responses to pathogenic and beneficial bacteria with Plant Growth Promoting effects (PGPB), which processes or specialized metabolites are involved in these responses and which regulatory proteins control these responses. This will be achieved through a multi-omics approach for which I will generate nuclear proteome data through protein proximity labelling, RNA-seq and metabolite data from A. thaliana seedlings exposed to a pathogen versus a PGPB. The second objective of this research will be to investigate the response regulation to a PGPB and a pathogen in specific root cell types by generating cell type specific nuclear proteome data. As the third objective I will determine the role of specific regulatory protein candidates from objectives 1 and 2 and their target cell type specific processes such as specialized metabolism or root development through individual gene by gene experiments. The proposed research will provide new insights into how plant responses to ‘friend or foe’ are regulated in different cell types. The generated datasets will be valuable for the community and provide a starting point for my own future independent research career.

Coordinador

MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV
Aportación neta de la UEn
€ 162 806,40
Dirección
HOFGARTENSTRASSE 8
80539 Munchen
Alemania

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Región
Bayern Oberbayern München, Kreisfreie Stadt
Tipo de actividad
Research Organisations
Enlaces
Coste total
€ 162 806,40