Descripción del proyecto
Vesículas extracelulares como fuente para el análisis de la inestabilidad de microsatélites en el cáncer de endometrio
El cáncer de endometrio es el cáncer ginecológico más común, y es necesario desarrollar tratamientos y medios de diagnóstico temprano eficientes. La inmunoterapia mejora las tasas de supervivencia en diversos tipos de cáncer, pero requiere unos biomarcadores fiables para la selección de los pacientes. La inestabilidad de microsatélites ha sido identificada como un candidato de biomarcador para la selección en la inmunoterapia. La evaluación de la inestabilidad de microsatélites requiere material genético del tumor, que puede obtenerse de las vesículas extracelulares del torrente sanguíneo. El proyecto EVOLVE, financiado por las Acciones Marie Skłodowska-Curie, se centrará en el desarrollo del enriquecimiento y análisis de vesículas extracelulares procedentes de fluidos corporales de pacientes con cáncer de endometrio y, así, evaluar la inestabilidad de microsatélites con un laboratorio en un chip y métodos de microfluídica, en combinación con biomarcadores de ADN y espectrometría Raman de superficie mejorada.
Objetivo
Endometrial cancer (EC) is the most common gynaecological cancer, and has an estimated incidence rise of more than 50% worldwide by 2040, mainly associated with increasing obesity prevalence. In 2018 alone, there were approximately 380,000 new cases and close to 90,000 deaths attributed to EC worldwide. Thus, there is a growing need for the development of better platforms for inexpensive, early stage EC diagnosis and treatments. Immunotherapy has shown a sharp increase in survival rate in various cancers, but only in a small percentage of treated patients. The lack of reliable biomarkers for the selection of this sub-group of patients is a great hindrance to its broader application in the clinical setting. However, microsatellite instability (MSI) has been shown to be a good candidate for immunotherapy selection. MSI evaluation requires genetic material from tumors, which can be found in extracellular vesicles released by EC cells to the bloodstream. “Isolation and Analysis of Extracellular Vesicles using OptofLuidics for the eValuation of Microsatellite Instability in Endometrial Cancer (EVOLVE)” is thus a project focused on the development of an integrated solution for the enrichment and analysis of extracellular vesicles from body fluids of endometrial cancer patients to assess microsatellite instability using lab-on-chip, microfluidics methods in conjunction with DNA biomarkers and surface-enhanced Raman scattering technology.
Ámbito científico
- engineering and technologyother engineering and technologiesmicrotechnologylab on a chip
- natural sciencesbiological sciencescell biology
- medical and health sciencesclinical medicineoncology
- medical and health sciencesbasic medicineimmunologyimmunotherapy
- medical and health scienceshealth sciencesnutritionobesity
Palabras clave
Programa(s)
- HORIZON.1.2 - Marie Skłodowska-Curie Actions (MSCA) Main Programme
Régimen de financiación
HORIZON-TMA-MSCA-PF-EF - HORIZON TMA MSCA Postdoctoral Fellowships - European FellowshipsCoordinador
4715-330 Braga
Portugal