Skip to main content
European Commission logo
polski polski
CORDIS - Wyniki badań wspieranych przez UE
CORDIS
CORDIS Web 30th anniversary CORDIS Web 30th anniversary
Zawartość zarchiwizowana w dniu 2024-06-18

Single Cell Imaging of Gene Activation during Oxidative Neuron Death: Towards Quantitative Systems Approaches

Cel

Ischaemic stroke and neurodegenerative disorders are leading causes of death and disability and their incidence is rising rapidly due to increases in European life expectancy. Excessive apoptosis is pathogenic when it concerns post-mitotic cells such as neurons; for instance in stroke or neurodegenerative diseases. Oxidative stress is a major contributor to ischemic and neurodegenerative processes; however, the molecular mechanisms and biological switches that determine outcome, i.e. neuronal tolerance or neuronal apoptosis, in response to oxidative stress are not fully characterised. This aim of this project is to analyse these events at the single cell level. The applicant has an excellent track record in the molecular analysis of pro-apoptotic gene activation in response to oxidative stress. Here, we propose to monitor and quantify the activation of cytoprotective (ARE- and HSE-containing) vs. pro-apoptotic (BIM) gene expression in neurons and astrocytes in real-time using GFP-based reporter assays and employing quantitative confocal and epifluorescence microscopy approaches. The quantitative single-cell data obtained will be incorporated into mathematical models of apoptosis activation developed by the host laboratory. The suggested fellowship will advance our understanding of this critically important biological process by allowing for a quantitative analysis of this signalling/gene activation network. The fellow will strongly complement the research activities of the host laboratory, and most importantly, will acquire new skills and be exposed to a unique interdisciplinary training programme. Furthermore, this work could provide important information for the development of new therapeutic strategies for ischemic and neurodegenerative disorders.

Zaproszenie do składania wniosków

FP7-PEOPLE-IEF-2008
Zobacz inne projekty w ramach tego zaproszenia

Koordynator

ROYAL COLLEGE OF SURGEONS IN IRELAND
Wkład UE
€ 177 354,02
Adres
ST STEPHEN'S GREEN 123
2 Dublin
Irlandia

Zobacz na mapie

Region
Ireland Northern and Western Border
Rodzaj działalności
Higher or Secondary Education Establishments
Kontakt administracyjny
Gayle Kenney (Dr.)
Linki
Koszt całkowity
Brak danych