CORDIS - Forschungsergebnisse der EU

Imaging flow cytometry using holographic virtual staining


Neue Plattform für bildgebende Durchflusszytometrie ohne Fluoreszenzmarker

Durchflusszytometer sind leistungsstarke Instrumente zur Hochdurchsatz- und multiparametrischen Analyse biologischer Zellen und weisen Einzelzelleigenschaften mittels Fluoreszenzfärbung nach. Die bildgebende Durchflusszytometrie integriert Bildgebungsfunktionen in Durchflusszytometer, um die Morphologie Tausender Zellen in heterogenen Zellpopulationen zu analysieren. Hierauf soll das Projekt HoloStain nun aufbauen. Mit der bildgebenden Durchflusszytometrie von HoloStain entfällt das zeitaufwändige und arbeitsintensive Einfärben der Zellen. Auf holographischen Bildern wird der Brechungsindex von Zellen dargestellt, und maschinelles Lernen ermöglicht das virtuelle Einfärben markierungsfreier Proben. HoloStain soll die bildgebende Durchflusszytometrie vereinfachen, Kosten senken und das für hohe Genauigkeit und Wiederholbarkeit erforderliche Know-how verringern, um dieses leistungsstarke Screening- und Diagnoseinstrument für die Praxis zugänglicher zu machen.


Imaging flow cytometry (IFC) holds great promise as clinical screening and diagnostics platform, as it can capture, process and analyse a large number of cells in bodily fluids, useful for hematology and hemato-oncology. However, currently IFC captures cell morphology by imaging each fluorescently-stained cell organelle on a different region of the camera sensor, dictating complicated and expensive optics and processing units. Current IFC remains a highly inaccessible technology, due to its very high cost, lack of accuracy, required operator expertise, and lack of objectiveness. The global flow cytometry market is estimated as USD 3.29 Billion in 2017, and projected to reach USD 4.79 Billion by 2022. This rapid growth is due to anticipation of becoming more common in clinical use. In this project, we will develop and commercialize a novel IFC platform. Using an external portable interferometric module and novel analysis methods, we perform quantitative holographic virtual staining (HoloStain) of the multiple cell organelles, simultaneously on a single IFC channel, dramatically simplifying the optics, cameras, and processing. Preliminary results are provided. As HoloStain does not require cell staining for morphological evaluation, it is ideal for real-time sorting or subsequent cell use. Moreover, HoloStain provides multiple virtually stained slices from the same camera frame, earning higher throughput. These unique advantages are expected to revolutionize the IFC market in two possible product-realization pathways: (i) as a more cost-effective stand-alone IFC workstation, and (ii) as an add-on module to an existing IFC workstation, upgrading its capabilities. Both pathways are highly attractive for licensing to existing flow cytometry manufacturers or for founding a startup company. In this project, upon completing marketing requirement and product characterizations, we will build an alpha prototype, and present it to potential strategic partners and investors.

Gastgebende Einrichtung

€ 150 000,00
69978 Tel Aviv

Auf der Karte ansehen

Higher or Secondary Education Establishments
€ 150 000,00

Begünstigte (1)