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DNA, Environment, Mineral Association: Reaction Kinetics

Descripción del proyecto

Mejora del método de ADN ambiental como herramienta de análisis genético

Varios organismos liberan ADN en el agua y los sedimentos, lo que sirve como un medio indirecto para detectar organismos presentes y pasados sin necesidad de muestrear tales organismos. El proyecto DENMARK, financiado con fondos europeos, aborda el estudio de las condiciones que protegen las moléculas de ADN y reducen su tasa de degradación. Los científicos del proyecto investigan las asociaciones entre ADN y minerales en varias muestras terrestres, de agua dulce y marinas, centrándose en particular en los enlaces y su estabilidad. Los resultados mejorarán la extracción y el análisis de ADN ambiental de muestras minerales, así como su utilización como fuente de información para estudios genéticos y de migración del ADN.

Objetivo

Environmental DNA (eDNA) is trace amounts of DNA released by organisms into water and sediments. It has recently attracted a lot of attention as it can be a valuable tool for detecting present and past organisms without the need to directly sample them. However, biotic and abiotic decay has severe effects on DNA survival. The adsorption of DNA to mineral surfaces in sediments as well as saline conditions have been shown to protect DNA molecules and decrease the DNA decay rate. Understanding which minerals and environmental conditions provide the best preservation of adsorbed DNA can thus allow us to: a) target such environments for paleoecological and palaeogenetic studies; b) serve as a quantitative tool for assessing DNA migration, i.e. is the recovered eDNA autochthonous?; and c) significantly advance eDNA extraction and analysis protocols by targeting and extracting eDNA from specific minerals.
Currently, we only have a qualitative understanding of the DNA-mineral adsorption/desorption at the bulk level and we have no quantitative insight into the DNA-mineral interactions and solution conditions that enhance DNA survival, nor the combined impact on DNA longevity in natural environments. With DENMARK I will (1) assess DNA-mineral associations and correlate with eDNA preservation state in sediments from terrestrial, freshwater and marine settings. Additionally, for a range of sedimentary minerals and a range of environmentally relevant solution compositions I will: (2) quantify the Gibbs free energy (ΔGbu) and kinetic bond parameters of in vitro DNA-mineral interactions; and (3) examine the nature, fraction and stability of interacting bonds of in vitro DNA-mineral associations at the bulk level. (4) Finally, I will link the sediment data to the in vitro single bond and bulk data to make a conceptual model for addressing the characteristics and longevity of different DNA-mineral systems, and improve eDNA extraction protocols.

Régimen de financiación

MSCA-IF-EF-ST - Standard EF

Coordinador

KOBENHAVNS UNIVERSITET
Aportación neta de la UEn
€ 219 312,00
Dirección
NORREGADE 10
1165 Kobenhavn
Dinamarca

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Región
Danmark Hovedstaden Byen København
Tipo de actividad
Higher or Secondary Education Establishments
Enlaces
Coste total
€ 219 312,00