Objective
The specific objective of this project is to study the factors determining the ability of a haem complex to bind, activate and specifically transform compounds. These factors may be classified as:
1) Structural (e.g. the nature of the available coordination site on the metal, type of donor atoms, electron density at the metal atom, superstructure of ligands, and steric effects);
2) Micro-environmental (e.g. polarity, hydrogen bonding ability of solvent, local pH and dynamics);
3) Mechanistic (e.g. competing and/or subsequent reactions, resistance to radical induced inactivation, relative binding affinities of substrate and inhibitor to the haem site).
- An engineered lignin peroxidase cDNA was overexpressed in E. coli. The mechanism of HRPC was studied.
- Microperoxidases which catalyse peroxidase-type and industrially relevant P450-type bioconversions were obtained.
- The structural effect of calcium in the enzymatic mechanism of LIP and HRP was studied.
- The crystal structure of a protein (cytochrome c6) was for the first time obtained by ab initio methods.
- The first 3D structure in solution of a paramagnetic haem protein was obtained. New NMR methods were developed.
- Electronic to protonic-energy was for the first time achieved in aqueous solution.
- Compounds with specific peroxidase activity were synthesised.
- Project deliverables:
1) New recombinant haem enzymes.
2) Development of first generation biomimetic Mn oxidation compounds.
3) Novel molecular calculation method developed.
4) Development of a model for homotropic (e{-}/e{-}) and heterotropic (e{-}/H{+}) cooperativities.
5) Development of new NMR methods.
6) Development of new methods for X-ray structural determinations.
Fields of science
- engineering and technologymaterials engineeringcrystals
- natural scienceschemical scienceselectrochemistryelectrolysis
- natural scienceschemical sciencesinorganic chemistryalkaline earth metals
- natural scienceschemical sciencescatalysis
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsenzymes
Topic(s)
Data not availableCall for proposal
Data not availableFunding Scheme
CSC - Cost-sharing contractsCoordinator
2780 Oeiras
Portugal
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Participants (5)
75270 Paris
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NR4 7UH Norwich
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50121 Firenze
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BN1 9QG Brighton
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6703 HA Wageningen
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