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INTELLIGENT BIOANALYTHICAL OPTICAL MONITORING SYSTEM FOR BIOTECHNOLOGICAL PROCESS DESIGN AND CONTROL

Objective


2D fluorescence spectroscopy was introduced as a new method for bioprocess monitoring. It is a useful tool for on-line monitoring and controlling biotechnological processes. It offers an insight into the bioprocess and the cell metabolism.

In contrast to conventional optical sensors, this sensor offers for the first time the possibility to monitor on-line several fluorophores in a cultivation process. Thus, it is possible to monitor simultaneously different variables of a bioprocess. The product concentration increase can be monitored simultanously with the cell concentration increase and the actual metabolic state of the cell as indicated by the cultivation experiments. A main problem of the conventional fluorosensors was that an overlapping of fluorophores could not be detected. This problem is overcome by the BioView sensor, which offers the possibility to monitor the whole spectral range simultaneously and opens tremendous application potential for the sensor. The sensor is a source of detailed process data, which of course must be interpreted by appropriate data handling. However, even the pure 2D spectra give a noninvasive insight into bioprocesses which is urgently needed for better bioprocess understanding and control. The different cultivation applications should show the potential of this method.
We propose to solve a very basic problem in bio technological process design and control by combining the newest optical methods using microbial process knowledge and model based datatreatment in an intelligent, non-invasive, on-line multicomponent fluorescence sensor.Today, process optimization is primarily empirical, and process control is often suboptimal due to the lack of on-line monitoring of the most important process state variables.The few existing sensors are simple single channel systems that are not optimized for turbid media and that lack essential combination of multichannel fluorescence, NIR and data interpretation software.It will be a huge step forward to be able to perform knowledge-based design, optimization and control with the multicomponent sensor described in this proposal.The major benefit of the development will be for process control and product quality in biotechnological processes leading to improve process economics and lover social and ecological loads.

This sensor represents generic technology applicable to many different fields, e.g. control of waste water treatment plants, biological gas plants, protein refinement, brewery etc.The main test platform for the sensor development will be fermentation processes, extended in the third year of the project to other systems.

Four industries and a university in three countries will solve the task together in a multidisciplinary collaboration.Each will contribute with know-how from different fields giving a symbiotic effect to strengthen the European cohesion and comtetitiveness within a field where Japan and USA today have strong positions.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

LYS & OPTIK
Address
Hjortekaersvej 99
2800 Lyngby
Denmark

Participants (5)

Dr Karl Thomae GmbH
Germany
Address
Birkendorfer Straße 65
7950 Biberach
NOVO NORDISK A/S
Denmark
Address
Novo Alle
2880 Bagsvaerd
OPTIMUM LTD
Greece
Address
Aristotelous Str. 177
11251 Athens
Universität Hannover
Germany
Address
Callinstraße 3
30167 Hannover
Westfälische Wilhelms-Universität Münster
Germany
Address
Wilhelm-klemm-straße 2
48149 Münster