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Validation of sediment toxicity tests for poorly water-soluble substances

Objective

- Development and interlaboratory calibration of standard methods for assessing the long-term effects of sediment-bound insoluble chemical substances to marine and freshwater invertebrate species
- Validation of standard laboratory methods towards field conditions
- Preparation of draft standard guidelines for sediment toxicity tests of poorly water-soluble substances


The project is a continuation of the two-year project STEP/MAST-CT91-0080 which aimed to develop and intercalibrate short-term lethal toxicity test methods for the assessment of sediment-bound insolubles.
This project will develop sublethal test methods and will validate in the field lethal and sublethal methods developed during the two phases of the project.
Three laboratories will develop, standardise, intercalibrate and validate a sublethal marine test, using an arthropode species such as Corophium volutator, three laboratories will devote their efforts to a freshwater sediment test using a chironomid species such as Chironomus riparius, and three will investigate a freshwater bivalve species such as Sphaerium corneum. Sub-lethal endpoints will include growth, reproduction and the induction of stress protein and detoxification enzymes. The effects of sediment-bound contaminants on bacterial genotoxicity will be investigated. A method developed in phase 1 of the project for assessing sediment mutagenicity will be further developed, intercalibrated and validated.
The two model substances to be spiked into natural, well-characterized sediments will be selected among the previously tested compounds (benzo-(a)-pyrene, PCB-118, HCH).

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

WRC plc
Address
Henley Road Medmenham
SL7 2HD Marlow - Buckinghamshire
United Kingdom

Participants (5)

INSTITUTE FOR INLAND WATER MANAGEMENT AND WASTE WATER TREATMENT
Netherlands
Address
Zuiderwagenplein 2
8200 AA Lelystad
Instituto Nacional de Investigação das Pescas
Portugal
Address
Avenida Brasilia
1400 Lisboa
MINISTRY OF TRANSPORT PUBLIC WORKS AND WATER MANAGEMENT
Netherlands
Address
Kortenaerkade 1
2500 EX Den Haag ('S-gravenhage)
Rijksuniversiteit Utrecht
Netherlands
Address
8,Padualaan
3584 CH Utrecht
UNIVERSITY OF HAMBURG
Germany
Address
Zeiseweg 9
22765 Hamburg