Objective
Four research teams (two from EC and two from NIC) are intended to study the participation of Ca2+ signalling in phytochrome transduction and its relation to other signalling pathways. The aim of the research is to determine the source of Ca2+ participating in phytochrome transduction which is expected to be vacuole, to evaluate the connection of phosphoinositide metabolism with light-induced Ca2+ release into cytoplasm, to study the heterogeneity of Ca2+ pool to be under control in plant cell of intercellular stimuli and to definite the state of phytochrome (soluble and membrane-bound) which triggers Ca2+ release.
Using the transgenic plants expressing the photoprotein aequorin in cytosolic and microdomain manners the collaborative research will be done in three direction:
1. Aequorin chemiluminescence measurements in the entire plants and protoplasts of wild and transgenic plants under the action of red light without and with pre-treatment with the inhibitors of phosphoinosotide metabolism and other inducers of Ca2+ release into cytoplasm as cyclic mononucleotides;
2. Ca2+ imagination measurements for exactly the same samples as it is indicated in 1;
3. Fluorescence probe luminescence measurements in protoplasts isolated both from the leaves of wild and transgenic (phyA, phyAOE) Arabidopsis.
It is expected to indicate the definite store for Ca2+ to be released under the action of light absorbed by phytochrome and to determine the Ca2+ translocation mechanisms responsible for the release, to show the relationship of phytochrome transduction to other signalling pathways in plant cell which are operating and summarised as the collaborative articles to be published in the scientific journals. An account will also be written.
Topic(s)
Call for proposal
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80638 Munchen
Germany