Objective
Regulation of gene expression in eukaryotic cell is directly involved in such biological processes as cell proliferation and differentiation. Recently found group of multifunctional proteins of Y-box protein family controls gene expression at the level of mRNA transcription in the nucleus and at the level of its translation in the cytoplasm. In eukaryotes and bacteria these proteins have been described as transcription factors of the Y-box containing genes. In Heidelberg and in Pushchino these proteins have been also identified as major proteins of cytoplasmic mRNPs affecting mRNA activity in translation. It is assumed that proteins of this family can affect DNA and mRNA template activity through dramatic changes in their structures. In spite of a remarkable progress in our knowledge about Y-box proteins in recent years, many question are not answered. Answers to some of them are planned to be obtained in the present project.
Intracellular distribution of these proteins will be studied using a number of techniques elaborated by the participants. Binding sites of Y-box proteins on giant polytene chromosomes will be localized. The stage of mRNA life story when it becomes associated with Y-box proteins will be determined as well as localization of these proteins in giant mRNPs and polyribosomes. It is also planned to study how functional state of the cell and phosphorylation of Y-box proteins affects their intracellular distribution and mRNP structure. Besides, cloning and sequencing of Y-box protein cDNA of Chironomus salivary gland cells and localization of genes for these proteins on polytene chromosomes will be performed.
Realization of this Project will allow to better understand functions of Y-box proteins in a cell, the mechanism of gene regulation and mRNP structural organization. The results obtained are planned to be published as joint papers.
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17177 Stockholm
Sweden