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Pure polychlorinated dibenzo-p-dioxins RMs


Industrial and urban wastes contain organo-chlorinated by-products, the incineration of which may result in release into the atmosphere significant amounts of toxic substances such as dioxin and benzofuran derivatives.
Some of these compounds such as 2, 3, 7, 8 TCDD are extremely toxic and must be readily identified and quantified in environmental samples containing hundreds of other chlorinated substances and in foodstuff, eg milk and cheese. There is an urgent need to develop analytical methods which not only allow identification and quantification at ppb levels, but also provide clues to the source of contamination by means of the particular pattern of isomer distribution. Such methods generally require extensive clean up to avoid interference of other chlorinated compounds.
The objective of the project is to prepare reference solutions of pure dioxins congeners which could be easily handled by laboratories for calibrating their analytical methods.


A procedure for the gravimetric preparation of a toxic solution has been developed. Four pure PCDDs have been specially synthesized with a purity of at least 98% and used for the gravimetric preparation of nonane reference solutions are available as internal standard for trace analysis. Twelve other toxic (2, 3, 7, 8 position) TCCD (dioxins) and TCD (furans) solutions were prepared with commercially available pure substances. The purity of the compounds was weighed in two different laboratories.


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Biochemisches Institut für Umweltcarcinogene
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Sieker Landstraße 19
22926 Ahrensburg

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