Alanine aminotransferase (ALT) is mainly located in the liver cells. This enzyme is normally present in serum but its concentration increases in cases of hepatic injuries. In acute hepatitis, ALT is the first enzyme with increased activity in serum. ALT can also be a useful indicator of the effect of drugs on the liver. With that of aspartate aminotransferase, ALT determination is the most frequent of the enzymatic tests performed in clinical chemistry laboratories.
The project consists in preparing a partially purified ALT from pork heart. Stability of the isolated enzyme will be assessed before the certification of the catalytic concentration of ALT can start. The method used for the ALT measurement is the IFCC recommended method.
Following a preliminary study that showed that a stable ALT could be prepared, the certification campaign was completed in 1991, on a new batch of 2000 samples. 13 laboratories participated and the following value and its uncertainty are proposed for certification:
CRM 426 (129 +/- 4) U/l.
The report describing the preparation and the certification exercise is being finalized for publication.
Funding SchemeCSC - Cost-sharing contracts
EN6 3QG Potters Bar