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ALANINE AMINOTRANSFERASE

Objective



Alanine aminotransferase (ALT) is mainly located in the liver cells. This enzyme is normally present in serum but its concentration increases in cases of hepatic injuries. In acute hepatitis, ALT is the first enzyme with increased activity in serum. ALT can also be a useful indicator of the effect of drugs on the liver. With that of aspartate aminotransferase, ALT determination is the most frequent of the enzymatic tests performed in clinical chemistry laboratories.

The project consists in preparing a partially purified ALT from pork heart. Stability of the isolated enzyme will be assessed before the certification of the catalytic concentration of ALT can start. The method used for the ALT measurement is the IFCC recommended method.

RESULTS

Following a preliminary study that showed that a stable ALT could be prepared, the certification campaign was completed in 1991, on a new batch of 2000 samples. 13 laboratories participated and the following value and its uncertainty are proposed for certification:
CRM 426 (129 +/- 4) U/l.
The report describing the preparation and the certification exercise is being finalized for publication.

Funding Scheme

CSC - Cost-sharing contracts

Coordinator

Centre du Médicament
Address

Nancy
France

Participants (13)

Boehringer Mannheim GmbH
Germany
Address

Penzberg
Centre Hospitalier Universitaire Vaudois
Switzerland
Address

Lausanne
Fondazione Centro San Romanello del Monte Tabor
Italy
Address

201 Milano
HANNOVER MEDICAL SCHOOL
Germany
Address
Carl-neuberg-strasse 1
30625 Hannover
Hôpital Necker - Enfants Malades
France
Address
149 Rue De Sèvres
75743 Paris
Hôpital de la Chaux-de-Fonds
Switzerland
Metaxas Memorial Cancer Hospital
Greece
Address

Piraeus
National Institute for Biological Standards and Control (NIBSC)
United Kingdom
Address
Blanche Lane South Mimms
EN6 3QG Potters Bar
Odense University Hospital
Denmark
Sint Radboud Ziekenhuis
Netherlands
Address

Nijmegen
Universidad Autonoma de Barcelona
Spain
University of Thessaloniki
Greece
Université de Strasbourg I (Université Louis Pasteur)
France
Address

67070 Strasbourg