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Stabilizing the exposure of neutralization epitopes on HIV-1 envelope glycoprotein trimer vaccines

Obiettivo

The impact of HIV/AIDS on world healthcare is tremendous, particularly in the Third World. To curtail the HIV epidemic a cheap and effective vaccine is urgently needed, but despite massive research efforts no vaccine is available yet. Although most vaccines work by inducing neutralizing antibodies, HIV has evolved many ways to limit the induction and binding of neutralizing antibodies. The challenge is to engineer Env subunit vaccines that do induce neutralizing antibodies efficiently. One aspect that has been highly underappreciated is conformational heterogeneity of Env. Conformational flexibility is exerted at three different levels. First, flexible variable loops and N-glycans protruding from the conserved protein core cause “local flexibility” at the protein surface. Second, movement between the conserved inner and outer domain of gp120 causes “tertiary flexibility”. Third, movement of the three gp120 protomers in the trimeric complex, resembling a flower that opens and closes, causes “quaternary flexibility”. These three levels of flexibility provide very unstable targets for recognition by low affinity B cell receptors on naïve B cells, diminishing the chance of efficient B cell activation and the secretion of neutralizing antibodies. Using a number of novel structure-based vaccine design strategies that include the introduction of stabilizing disulfide bonds, we intend to remove the undesirable flexibility on Env trimers to provide a homogeneous and stable target to B cells. This should result in stabilized Env immunogens that are better in inducing neutralizing antibodies compared to the current state-of-the art Env vaccines. This is a highly interdisciplinary project on the crossroads of immunology and protein chemistry and should result in protein immunogens that elicit improved neutralizing antibody responses against HIV and should provide answers to fundamental questions on how B cells “see” protein immunogens.

Invito a presentare proposte

ERC-2011-StG_20101109
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Meccanismo di finanziamento

ERC-SG - ERC Starting Grant

Istituzione ospitante

ACADEMISCH MEDISCH CENTRUM BIJ DE UNIVERSITEIT VAN AMSTERDAM
Indirizzo
Meibergdreef 15
1105AZ Amsterdam
Paesi Bassi
Tipo di attività
Higher or Secondary Education Establishments
Contributo UE
€ 1 499 943
Ricercatore principale
Rogier Willem Sanders (Dr.)
Contatto amministrativo
Gulseren Yalvac (Ms.)

Beneficiari (1)

ACADEMISCH MEDISCH CENTRUM BIJ DE UNIVERSITEIT VAN AMSTERDAM
Paesi Bassi
Contributo UE
€ 1 499 943
Indirizzo
Meibergdreef 15
1105AZ Amsterdam
Tipo di attività
Higher or Secondary Education Establishments
Ricercatore principale
Rogier Willem Sanders (Dr.)
Contatto amministrativo
Gulseren Yalvac (Ms.)