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High-Density Peptide MicroArrays and high-throughput, label-free detection of peptides, modifications and interactions

Objective

Proteins are extremely malleable building blocks of life involved in all aspects of biology. Many diseases are caused by proteins aberrations, and proteins are frequent targets of intervention. Mapping all proteins and their functions are expected to yield pervasive medical and biotechnological benefits. However, even the most comprehensive and high-throughput proteins discovery technologies are seriously challenged by the extreme diversity and low abundance of many proteome components; a problem, compounded by the lack of affinity reagents and validated probes for sample preparation and identification. Our concept is that shorter protein fragments, or peptides, may offer solutions to many of these problems as peptides may represent or mimic proteins. Using in situ solid-phase peptide synthesis, computerized photolithography and novel photochemistry, we have recently generated peptide microarrays of up to 2 million addressable peptides. This unprecedented high-density and high-content peptide microarray technology could make inroads into the kind of high-throughput analysis needed to address the entire human proteome. Here, we aim to exploit this potential by using and improving three different, yet complementary, label-free detection technologies allowing sensitive, high-resolution determinations of the identity, quality and/or modification of individual members of a peptide microarray, and real-time monitoring of any interacting molecular receptor. We will also develop peptides as rapid, specific, and renewable affinity reagents for complex sample preparation, and develop peptides as probes and complex biosensors. Three SME’s constitute the backbone of this collaboration, receiving 50% of the budget, and enjoying significant opportunities from the booming protein/peptide microarray market. Furthermore, solutions to these unmet needs of proteomics are believed to have incalculable benefits for European health, innovativeness and competiveness.
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Coordinator

KOBENHAVNS UNIVERSITET

Address

Norregade 10
1165 Kobenhavn

Denmark

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 717 609,85

Administrative Contact

Ivan Kristoffersen (Mr.)

Participants (9)

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SCHAFER -N APS

Denmark

EU Contribution

€ 1 061 166,80

IMABIOTECH SAS

France

EU Contribution

€ 658 300

PLASMORE SRL

Italy

EU Contribution

€ 697 860

JRC -JOINT RESEARCH CENTRE- EUROPEAN COMMISSION

Belgium

EU Contribution

€ 316 510,80

FRAUNHOFER GESELLSCHAFT ZUR FOERDERUNG DER ANGEWANDTEN FORSCHUNG E.V.

Germany

EU Contribution

€ 147 831

BRUKER DALTONIK GMBH

Germany

EU Contribution

€ 148 817,50

THE UNIVERSITY OF MANCHESTER

United Kingdom

EU Contribution

€ 314 070

UNIVERSITY OF BATH

United Kingdom

EU Contribution

€ 444 300,80

DANMARKS TEKNISKE UNIVERSITET

Denmark

EU Contribution

€ 316 547,25

Project information

Grant agreement ID: 278832

Status

Closed project

  • Start date

    1 January 2012

  • End date

    30 April 2016

Funded under:

FP7-HEALTH

  • Overall budget:

    € 6 382 419,95

  • EU contribution

    € 4 823 014

Coordinated by:

KOBENHAVNS UNIVERSITET

Denmark