CORDIS
EU research results

CORDIS

English EN

Bottom-up reconstitution of a biomimetic bioartificial liver

Objective

The liver, by way of its central role in both endogenous and exogenous metabolism, is one of the most well-studied organs in the human body.
Hepatic tissue and its derivatives have a wide range of in-vivo and in-vitro applications from whole organ or partial lobe transplant, bioartificial ex-vivo devices, treatment of metabolic disorders to toxicology, drug metabolism and tissue regeneration. On the one hand, suitable donor livers for solid organ transplant are in short supply, while chronic liver diseases are on the increase both in Europe and world-wide. On the other hand, in-vitro and ex-vivo technologies for recapitulating liver function still fall short of reliability, consistency and predictivity, precluding many commercial applications. There is a dire need for innovative and reproducible methods for developing functional bioartificial livers or portions of liver which can be easily transplanted or reliably integrated into extracorporeal devices, essential for treating acute liver failure and other metabolic liver disorders. To avoid the risks and complications associated with animal/human matrices, yet furnish a reliable and reproducible 3D microarchitecture capable of maintaining the detoxification and metabolic functions of healthy human liver, our aim is to fabricate a novel hepatic lobuli ECM replica seeded with stable human hepatocytes and endothelial cells using the human liver as a design template through a bottom up approach. These cell-containing bioartificial constructs will be developed and characterized in vitro to assess metabolic function, protein production and angiogenic potential. They will then be implanted in animal models through minimally invasive techniques as a solid organ transplant alternative to recombinant Factor VIII-based therapy for patients with haemophilia A.

Coordinator

UNIVERSITA DI PISA

Address

Lungarno Pacinotti 43/44
56126 Pisa

Italy

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 824 999

Administrative Contact

Immacolata Viva (Dr.)

Participants (4)

Sort alphabetically

Sort by EU Contribution

Expand all

THE ELECTROSPINNING COMPANY LTD

United Kingdom

EU Contribution

€ 559 356

MEDICYTE GMBH

Germany

EU Contribution

€ 1 738 180

THE UNIVERSITY OF MANCHESTER

United Kingdom

EU Contribution

€ 763 152

GABO:MI GESELLSCHAFT FUR ABLAUFORGANISATION:MILLIARIUM MBH & CO KG

Germany

EU Contribution

€ 349 190

Project information

Grant agreement ID: 304961

Status

Closed project

  • Start date

    1 July 2012

  • End date

    30 June 2015

Funded under:

FP7-HEALTH

  • Overall budget:

    € 5 377 150,80

  • EU contribution

    € 4 234 877

Coordinated by:

UNIVERSITA DI PISA

Italy