CORDIS
EU research results

CORDIS

English EN

Development of GlycoPhage Arrays as a novel high-throughput method for glycomics

Objective

Complex carbohydrates (glycans) are attached to proteins and lipids by the process of glycosylation and play important roles in many biological processes including cell-cell recognition, metabolic trafficking and host-pathogen interactions. Altered glycosylation or variations in the synthesis of glycans are known to cause diseases including cancer, retroviral infection and disorders of the heart, lung and blood. In order to establish connections between glycan structures and their functions (functional glycomics), to monitor glycosylation in disease diagnosis and prognosis, and to elucidate molecular mechanisms involved in pathogenesis, the development of precise, robust and sensitive methodologies for glycan analysis is critical. Carbohydrate-based arrays, or “glycoarrays,” have emerged in the last decade as a powerful tool, however to fully exploit the potential of arrays, it will be necessary to (i) increase the quantity and diversity of carbohydrate structures and (ii) develop reliable and reproducible chemistries for the immobilization of the carbohydrate probes onto solid support. Recently, the protein glycosylation locus (Pgl) discovered in Campylobacter jejuni was functionally transferred to E. coli, conferring ability to glycosylate proteins. Additionally, Dr. Celik has recently demonstrated glycosylation of phage particles simply by infecting the glycosylation competent E. coli with M13 phage displaying an acceptor protein. The hypothesis of this particular application is that the presentation of N-glycosylated proteins and O-antigens on phage particles can be exploited for the development of glycan arrays. The study will be significant because it will overcome the current bottlenecks in glycan array construction and provide a relatively inexpensive, specific and stable glycan representation method, as well as introduce a simplified and universal purification technique that is not dependent on the carbohydrate.
Leaflet | Map data © OpenStreetMap contributors, Credit: EC-GISCO, © EuroGeographics for the administrative boundaries

Coordinator

HACETTEPE UNIVERSITESI

Address

Hacettepe Universitesi Beytepe Kampusu Rektorluk Binasi
06800 Cankaya Ankara

Turkey

Activity type

Higher or Secondary Education Establishments

EU Contribution

€ 100 000

Administrative Contact

Zumriye Aksu (Prof.)

Project information

Grant agreement ID: 322096

Status

Closed project

  • Start date

    1 October 2012

  • End date

    30 September 2016

Funded under:

FP7-PEOPLE

  • Overall budget:

    € 100 000

  • EU contribution

    € 100 000

Coordinated by:

HACETTEPE UNIVERSITESI

Turkey