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Pre- and postsynaptic signaling at single synaptic contacts

Objective

"The main goal of the project is to develop new procedures to investigate single synaptic sites of the mammalian brain. For this purpose, synaptically connected pairs of molecular layer interneurons will be studied in cerebellar slices. Presynaptic calcium transients will be elicited at individual presumptive presynaptic release sites using targeted photorelease of the calcium precursor DM-nitrophen. Using this new approach several questions will be addressed including the degree of postsynaptic receptor saturation, the size and nature of the readily releasable pool of synaptic vesicles, and kinetics of vesicle recycling.
This approach will be first developed for the GABAergic synapses between interneurons but will be later extended to study the glutamatergic synapses between granule cells and interneurons.
Next we shall develop methods to selectively activate presynaptic ionotropic receptors by localized photolysis of precursors of neurotransmitters, while recording either the resulting current or the resulting calcium signal. In this manner we shall investigate, at the single site level, the presence and functional properties of presynaptic GABAA and glutamate receptors.
Finally we will investigate the functional significance of presynaptic receptor signaling in vivo by using calcium imaging with genetically targeted calcium indicators. Using appropriate stimulation protocols we will determine whether these receptors can be activated either via autocrine or paracrine release of neurotransmitters."
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Principal Investigator

Alain Noël Marty (Dr.)

Host institution

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS

Address

Rue Michel Ange 3
75794 Paris

France

Activity type

Research Organisations

EU Contribution

€ 2 494 480

Principal Investigator

Alain Noël Marty (Dr.)

Administrative Contact

Alain Mangeol (Mr.)

Beneficiaries (1)

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CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS

France

EU Contribution

€ 2 494 480

Project information

Grant agreement ID: 294509

Status

Closed project

  • Start date

    1 December 2012

  • End date

    30 November 2017

Funded under:

FP7-IDEAS-ERC

  • Overall budget:

    € 2 494 480

  • EU contribution

    € 2 494 480

Hosted by:

CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE CNRS

France