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Self-assembled virus-like vectors for stem cell phenotyping

Objective

The Self-Assembled Virus-like Vectors for Stem Cell Phenotyping (SAVVY) project relies on hierarchical, multi-scale assembly of intrinsically dissimilar nanoparticles to develop novel types of multifunctional Raman probes for analysis and phenotyping of heterogeneous stem cell populations.
Our project will address a large unmet need, as stem cells have great potential for a broad range of therapeutic and biotechnological applications. Characterization and sorting of heterogeneous stem cell populations has been intrinsically hampered by (1) lack of specific antibodies, (2) need for fluorescence markers, (3) low concentration of stem cells, (4) low efficiencies/high costs.
Our technology will use a fundamentally different approach that (1) does not require antibodies, aptamers, or biomarkers, (2) is fluorescence-label free, and (3) is scalable at acceptable cost. The approach uses intrinsic differences in the composition of membranes of cells to distinguish cell populations. These differences will be detect by SERS and analysed through multicomponent analysis.
We have combined the necessary expertise to tackle this challenge: Stellacci has developed rippled nanoparticles that specifically interact with and adhere to cell membranes (analogues to cell penetrating peptides). Lahann has developed bicompartmental Janus polymer particles that have already been surface-modified with rippled particles and integrate specifically in the cell membrane (analogues to viruses). Liz-Marzan has developed highly Raman-active nanoparticles and has demonstrated their selectivity and specificity in SERS experiments. These Raman probes will be loaded into the synthetic viruses to enable membrane fingerprinting. Stevens has developed a Bioinformatics platform for fingerprinting of stem cell populations using cluster analysis algorithms. The effort will be joined by two SMEs, ChipShop and OMT, that will be able to develop the necessary microfluidic and Raman detection hardware.

Call for proposal

FP7-NMP-2012-SMALL-6
See other projects for this call

Coordinator

KARLSRUHER INSTITUT FUER TECHNOLOGIE
Address
Kaiserstrasse 12
76131 Karlsruhe
Germany
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 067 915,08
Administrative Contact
Natascha Kindsvogel (Ms.)

Participants (7)

ECOLE POLYTECHNIQUE FEDERALE DE LAUSANNE
Switzerland
EU contribution
€ 509 714
Address
Batiment Ce 3316 Station 1
1015 Lausanne
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Francesco Stellacci (Prof.)
UNIVERSIDAD DE VIGO
Spain
EU contribution
€ 105 000
Address
Lg Campus Lagoas Marcosende
36310 Vigo Pontevedra
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Anxo Moreira González (Mr.)
IMPERIAL COLLEGE OF SCIENCE TECHNOLOGY AND MEDICINE
United Kingdom
EU contribution
€ 788 045,20
Address
South Kensington Campus Exhibition Road
SW7 2AZ London
Activity type
Higher or Secondary Education Establishments
Administrative Contact
Shaun Power (Mr.)
MICROFLUIDIC CHIPSHOP GMBH
Germany
EU contribution
€ 580 400
Address
Stockholmer Strasse 20
07747 Jena
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Holger Becker (Dr.)
OMT-OPTISCHE MESSTECHNIK GMBH

Participation ended

Germany
Address
Horvelsinger Weg 6
89081 Ulm
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Nancy Hecker-Denschlag (Dr.)
ASOCIACION CENTRO DE INVESTIGACION COOPERATIVA EN BIOMATERIALES- CIC biomaGUNE
Spain
EU contribution
€ 468 289
Address
Paseo Miramon 182, Parque Tecnologico De San Sebastian Edificio Empresarial C
20009 San Sebastian
Activity type
Research Organisations
Administrative Contact
Alfonso Egaña (Mr.)
WITEC WISSENSCHAFTLICHE INSTRUMENTEUND TECHNOLOGIE GMBH
Germany
EU contribution
€ 263 365,72
Address
Lise Meitner Strasse 6
89081 Ulm
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Administrative Contact
Joachim Koenen (Dr.)