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Assembly and function of the crenarchaeal flagellum

Ziel

"Archaea constitute the third domain of life and are believed to be close to the origin of life. They comprise a diverse group of micro-organisms that combine bacterial and eukaryotic features, but also employ many novel mechanisms. They possess a unique cell envelope with a cytoplasmic membrane of ether lipids surrounded by a proteinaceous S-layer and various cell appendages such as flagella, pili and more unusual structures. Studies have shown that the archaeal flagellum is an unique structure as it functionally resembles the bacterial flagellum, but structurally it is a simple type IV pilus. Moreover, we have shown that this type IV pilus can rotate. Therefore I propose to name the archaeal flagellum, the archaellum, as it is fundamentally different from the bacterial flagellum.

In this proposal I aim to understand the assembly and mechanism of rotation of the archaellum of the thermocacidophilic crenarchaen Sulfolobus acidocaldarius by using biochemical, genetic and biophysical methods. The main milestons are:

- Biochemical and structural characterization of all archaellum subunits
- To understand the assembly pathway of the archaellum and the interactions of its different
subunits
- To understand how rotation of the filament is achieved and which subunits are important
for this movement

This work will identify a new, relatively simple motor complex that has evolved from primordial type IV pili assembly machineries and therefore uncover general principles of macromolecular assemblies at cellular surfaces and a novel mechanism to generate mechanical force that can be translated into movement."

Aufforderung zur Vorschlagseinreichung

ERC-2012-StG_20111109
Andere Projekte für diesen Aufruf anzeigen

Gastgebende Einrichtung

ALBERT-LUDWIGS-UNIVERSITAET FREIBURG
Adresse
Fahnenbergplatz
79098 Freiburg
Deutschland
Aktivitätstyp
Higher or Secondary Education Establishments
EU-Beitrag
€ 1 165 700,40
Hauptforscher
Sonja-Verena Albers (Dr.)
Kontakt Verwaltung
Sonja-Verena Albers (Ms.)

Begünstigte (2)

ALBERT-LUDWIGS-UNIVERSITAET FREIBURG
Deutschland
EU-Beitrag
€ 1 165 700,40
Adresse
Fahnenbergplatz
79098 Freiburg
Aktivitätstyp
Higher or Secondary Education Establishments
Hauptforscher
Sonja-Verena Albers (Dr.)
Kontakt Verwaltung
Sonja-Verena Albers (Ms.)
MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV

Beteiligung beendet

Deutschland
EU-Beitrag
€ 298 616,66
Adresse
Hofgartenstrasse 8
80539 Munchen
Aktivitätstyp
Research Organisations
Kontakt Verwaltung
Regine Kahmann (Prof.)