Objetivo Chromatin undergoes fascinating structural and functional changes during the metazoan cell cycle. It massively condenses at the beginning of mitosis with a degree of compaction up to fiftyfold higher than in interphase. At the end of mitosis, mitotic chromosomes decondense to re-establish their interphase chromatin structure. This process is indispensable for reinitiating transcription and treplication, and is thus of central importance in the cellular life cycle. Despite its significance to basic research as well as its potential medical implications, postmitotic chromatin decondensation is only poorly understood. It has been well described cytologically, but we lack an understanding of the underlying molecular events. We are ignorant about the proteins that mediate chromatin decondensation, the distinct steps in this multi-step procedure and their regulation.Using a novel in vitro assay, which recapitulates the process in the simplicity of a cell free reaction, we will identify the molecular machinery mediating postmitotic chromatin decondensation and define the different steps of the process. The cell free assay offers the unique possibility to isolate and purify activities responsible for individual steps in chromatin decondensation, to identify their molecular composition and to analyse the molecular changes they induce on chromatin. Accompanied by live cell imaging in mammalian tissue culture cells, the proposed approach will not only facilitate the elucidation of the factors involved in chromatin decondensation, but will also provide insight into how this process is integrated into mitotic exit and nuclear reformation and linked to other concomitant processes such as nuclear envelope assembly or nuclear body formation.Thus, using an unprecedented approach to study the ill-defined but important cell biological process of postmitotic chromatin decondensation, we aim to expand the frontiers in our knowledge on this topic. Ámbito científico natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsnatural sciencesbiological sciencesgeneticschromosomes Programa(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Tema(s) ERC-SG-LS1 - ERC Starting Grant - Molecular and Structural Biology and Biochemistry Convocatoria de propuestas ERC-2012-StG_20111109 Consulte otros proyectos de esta convocatoria Régimen de financiación ERC-SG - ERC Starting Grant Institución de acogida UNIVERSITAETSKLINIKUM AACHEN Aportación de la UE € 474 738,58 Dirección Pauwelsstrasse 30 52074 Aachen Alemania Ver en el mapa Región Nordrhein-Westfalen Köln Städteregion Aachen Tipo de actividad Higher or Secondary Education Establishments Investigador principal Wolfram Antonin (Dr.) Contacto administrativo Volker Legewie (Mr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos Beneficiarios (2) Ordenar alfabéticamente Ordenar por aportación de la UE Ampliar todo Contraer todo UNIVERSITAETSKLINIKUM AACHEN Alemania Aportación de la UE € 474 738,58 Dirección Pauwelsstrasse 30 52074 Aachen Ver en el mapa Región Nordrhein-Westfalen Köln Städteregion Aachen Tipo de actividad Higher or Secondary Education Establishments Investigador principal Wolfram Antonin (Dr.) Contacto administrativo Volker Legewie (Mr.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos MAX-PLANCK-GESELLSCHAFT ZUR FORDERUNG DER WISSENSCHAFTEN EV La participación finalizó Alemania Aportación de la UE € 1 025 141,42 Dirección HOFGARTENSTRASSE 8 80539 Munchen Ver en el mapa Región Bayern Oberbayern München, Kreisfreie Stadt Tipo de actividad Research Organisations Contacto administrativo Antje Lemper-Rupp (Mrs.) Enlaces Contactar con la organización Opens in new window Sitio web Opens in new window Coste total Sin datos